Sulfate Beads-C: Advanced Affinity Chromatography for Virus and Protein Purification
Sulfate Beads-C offers a revolutionary approach to virus and protein purification. This affinity chromatography medium provides efficient concentration, purification, and depyrogenation of viruses, antigens, and heparin-binding proteins. It outperforms traditional methods in safety, yield, and scalability.
Key Features and Benefits
1
Wide Affinity Range
Binds diverse viruses, antigens, and heparin-binding proteins. Enables purification of various biological products in one step.
2
Enhanced Safety
Closed-column operation ensures sterility. Eliminates hazards associated with ultracentrifugation methods.
3
Improved Efficiency
Simultaneous concentration and purification. Reduces processing steps, time, and costs compared to traditional methods.
4
Scalability
Rigid beads allow high flow rates. Easily scalable from small-scale research to large-scale production.
Physical and Chemical Properties
Applications in Virus Purification
Enveloped Viruses
Effective for purifying rabies, influenza, and coronaviruses. Binds envelope proteins for efficient capture.
Viral Antigens
Purifies hepatitis B surface antigen and herpes simplex glycoproteins. Ideal for vaccine development.
Microbial Antigens
Captures filamentous hemagglutinin from B. pertussis. Useful in bacterial vaccine production.
Case Study: Human Coronavirus OC43 Purification
Sulfate Beads-C effectively purified human coronavirus OC43 from Vero cell lysate. A small column (5 mm x 15 mm) was used. Elution with high-salt buffer yielded concentrated, purified virus.
This demonstrates the beads' capability to bind and purify enveloped viruses. It highlights potential applications in coronavirus research and vaccine development.
Protein Purification Applications
1
Plasma Proteins
Purifies antithrombin III and complement factors. Useful in blood product manufacturing.
2
Growth Factors
Binds cellular growth factors. Applications in stem cell research and regenerative medicine.
3
Enzymes
Captures lipases and kinases. Potential use in industrial enzyme production.
Endotoxin Removal Capability
Sulfate Beads-C excels in depyrogenation of viral and bacterial preparations. It does not bind endotoxins, allowing their effective removal. This feature is crucial for producing safe vaccines and biotherapeutics.
In B. pertussis purification, endotoxin levels were reduced below standard levels. This was confirmed by Limulus lysate, rabbit pyrogen, and mouse toxicity tests.
Custom Resin Development Services
LINDHOLT BIOSCIENCE offers custom resin development. They optimize existing products and develop new applications. This service caters to both large and small customers in life sciences.
Researchers can collaborate to create tailored solutions. This ensures optimal performance for specific purification needs.